Flavor Analysis and Strain Exploitation of Fermented Rice Noodles

Di Chen, Zhengbiao Gu, Zhaofeng Li, Yan Hong, Li Cheng, Caiming Li

Abstract


Fermented rice noodles is popular for its unique flavor, but the study on flavor is lacking, especially the screening of corresponding strains. For the first time, the relationship of microorganism and flavor was unveiled, based on the research of a typical fermented rice noodles from Guilin in China. Flavors were dissected by HPLC-MS, GC-MS, GC-MS-O (Gas chromatography-mass and spectrometry-olfactometry) analyses, while microbial community was investigated by amplicon sequencing and metagenomics, afterwards microorganisms and flavors were connected via correlation analysis and function annotation, hen strains could be explored based on the analyses. Ethanol, 1-butanol,3-methyl-, 2-butanone, 3-hydroxy-, acetic acid and phenylethanol were discovered to be key flavors, biogenic amines were not abundant enough to have bad effect on flavor, but putrescine, tyramine and histamine produced in fermentation needed supervision for safety. Analysis indicated that fermentation was dominated by lactic acid bacteria. Further analysis revealed that Lactobacillus, Acetobacter and Lactococcus were primary creators for these key flavors. In particular, Lactobacillus showed great potential in flavor forming. Additionally, later experiment suggested that samples fermented by L. fermentum and L. plantarum (both belong to Lactobacillus) possessed great flavors, when L. fermentum also showed the ability to inhibit mold and reduce biogenic amine content.


Keywords


Food Science; Rice Noodles; Fermentation; High Throughput Sequencing; Flavor

Full Text:

PDF

Included Database


References


Tamang J P. Fermented Foods and Beverages of the World. New York: CRC Press, 2010.

Lu Z H, Cao W, Peng H H, et al. Effect of fermentation metabolites on rheological and sensory properties of fermented rice noodles. Journal of the Science of Food and Agriculture, 2008,88(12): 2134-2141.

Lu Z H, Cao W, Peng H H, et al. The effects of natural fermentation on the physical properties of rice flour and the rheological characteristics of rice noodles. International Journal of Food Science and Technology, 2005,40(9): 985-992.

Lu Z H, Li L L, Cao W, et al. Influence of natural fermentation on physico-chemical characteristics of rice noodles. International Journal of Food Science and Technology, 2003,38(5): 505-510.

Seo M J, Kang B W, Park J U, et al. Noodle Development and Its Quality Characteristics Using Fermented White and Brown Rice. Journal of Life Science, 2012,22(10): 1378-1383.

Min Weihong. Study on the mechanism of lactic acid bacteria fermentation to improve the edible quality of rice flour. Beijing: China Agricultural University, 2003.

Francesca De Filippis, Eugenio Parente, Danilo Ercolini. Recent Past, Present,and Future of the Food Microbiome. Annual Review of Food Science and Technology, 2018,9: 589-608.

Li Yun, the changes of bacterial phase during the production of fermented rice flour and the influence of fermentation on the quality of rice flour. Beijing: China Agricultural University, 2015.

Garcia-Armisen T, Papalexandratou Z, Hendryckx H, et al. Diversity of the total bacterial community associated with Ghanaian and Brazilian cocoa bean fermentation samples as revealed by a 16 S rRNA ge ne clone library. Applied Microbiology and Biotechnology, 2010. 87(6): 2281-92.

Wang S L, Wu Q, Nie Y, et al. Construction of synthetic microbiota for reproducible flavor metabolism in Chinese light aroma type liquor produced by solid-state fermentation. Food Microbiology, 2019,85(10): e03090-18.

Zhao Z, Mu T, Sun H. Microbial characterization of five Chinese traditional sourdoughs by high-throughput sequencing and their impact on the quality of potato steamed bread. Food Chemistry, 2019. 274: 710-717.

Wolfe B E, Button J E, Santarelli, et al. Cheese Rind Communities Provide Tractable Systems for In Situ and In Vitro Studies of Microbial Diversity. Cell, 2014,158(2): 422-433.

Zhang J C, Yang Y C, Deng J, et al. Dynamic profile of the microbiota during coconut water pre-fermentation for Nata de coco production. LWT - Food Science and Technology, 2017,81: 87-93.

Lu Z M, Liu N, Wang L J, et al. Elucidating and Regulating the Acetoin Production Role of Microbial Functional Groups in Multispecies Acetic Acid Fermentation. Applied and environmental microbiology, 2016,82(19): 5860-8.

Jiang Wenguang. Study on Free Aroma Compounds in Grape of Tonglongzhu from Yantai Production Area. Wuxi: Jiangnan University, 2008.

National Health and Family Planning Commission of the People's Republic of China ,GB 5009.208-2016, Determination of Biogenic Amines in National Food Safety Standards,. Beijing : China Standards Publishing House, 2016.

National Health and Family Planning Commission of the People's Republic of China, GB/T 10220-2012. General Review of Sensory Analysis Methodology, Beijing: National Standards Publishing House, 2012.

Li Z Y, Feng C X, Luo X G, et al. Revealing the influence of microbiota on the quality of Pu-erh tea during fermentation process by shotgun metagenomic and metabolomic analysis. Food Microbiology, 2018,76: 405-415.

Solieri L,Giudici P. Yeasts associated to Traditional Balsamic Vinegar: Ecological and technological features. International Journal of Food Microbiology, 2008,125(1): 36-45.

Shalaby A R. Significance of biogenic amines to food safety and huaman healthy. Food Research International, 1996,29(7): 675-690.

Park, Y K, Lee JH, Mah JH. Occurrence and reduction of biogenic amines in traditional Asian fermented soybean foods: A review. Food Chemistry, 2019,278: 1-9.

Regubalan B, Ananthanarayan L. Investigation of biogenic amines content in fermented idli batter during storage. Journal of Food Science and Technology, 2019,56(4): 1775-1784.

Xiong Qing, Fan Lu, Bao Fang et al. Flavor formation and characteristic analysis of rice cake. Food Science, 2011.32 (24): 232-236.

Liu N, Chen X, Sun Y et al. GC- MS Analysis of the Flavor of Traditional Sour Dough Steamed Bread and the Effect of Adding Edible Alkali on Its Flavor. Food Industry Science and Technology ,2014,16: 76-81.

Bruyn F D, Zhang S Y, Pothakos V, et al. Exploring the Impacts of Postharvest Processing on the Microbiota and Metabolite Profiles during Green Coffee Bean Production. Applied and environmental microbiology, 2017,83(1).

Lyu C Y, Chen C Y, Ge F, et al. A preliminary metagenomic study of puer tea during pile fermentation. Journal of Food Agriculture and Environment, 2013,93(13): 3165-74.

Park S E, Seo S H, Kim E J, et al. Changes of microbial community and metabolite in kimchi inoculated with different microbial community starters. Food Chemistry, 2019,274: 558-565.

Li Q, Fang F, Du G. Application of weissbrodt in fermented food. Food and Fermentation Industry, 2017,43(10): 241-246.

Tamang, J P, Watanabe K, Holzapfel W H. Review: Diversity of Microorganisms in Global Fermented Foods and Beverages. Front Microbiol, 2016,7: 377.

Tao Y, Wang X, Li X Z et al. The functional potential and active populations of the pit mud microbiome for 360 the production of Chinese strong-flavour liquor. Microbiol Biotechnology, 2017,10(6): 1603-1615.

National Health and Family Planning Commission of the People's Republic of China, GB 7099-2015, National Food Safety Standard Pastries and Bread. Beijing : China Standards Publishing House, 2016.

Cui L, Guo W. Bacteriostatic substances produced by lactic acid bacteria and their mechanism of action. Journal of Food Safety and Quality Testing, 2018, 9 (11):2578-2584.




DOI: https://doi.org/10.18686/fsr.v2i1.1318

Refbacks

  • There are currently no refbacks.