Phycobiliprotein content and growth profile of Spirulina platensis in airlift photobioreactor
Abstract
Microalgae are versatile organisms capable of adapting to diverse environmental conditions, allowing for enhanced biomass production and metabolite synthesis. Due to their rich biochemical composition and rapid growth rate, they are widely utilized across various industries. Microalgae cultivation can be conducted at different scales and methods, including photobioreactors and open ponds. Among various microalgae species, Spirulina platensis is particularly favored in industrial applications and recognized as one of the most significant commercial sources of phycobiliproteins, bioactive compounds unique to blue-green algae. Phycocyanin, a phycobiliprotein derived from S. platensis, is especially valued for its high-added economic potential. This study investigates the production of phycocyanin from S. platensis using an airlift photobioreactor, which offers advantages such as the elimination of mechanical mixing components and the efficient replacement of O2 and CO2 through aeration. The specific growth rate of S. platensis was determined to be 0.28 ± 0.01 day−1, with a doubling time of 2.47 ± 0.09 days. The phycobiliprotein concentrations were measured as follows: 1.03 ± 0.001 mg/L for phycoerythrin, 10.37 ± 0.08 mg/L for phycocyanin, 6.29 ± 0.24 mg/L for allophycocyanin, and 17.71 ± 1.76 mg/L for total phycobiliprotein. This study successfully cultivated S. platensis in an airlift photobioreactor, demonstrating its potential for efficient production of biomass and bioactive materials.
Keywords
Full Text:
PDFReferences
[1]Ahda M, Suhendra, Permadi A. Spirulina Platensis Microalgae as High Protein-Based Products for Diabetes Treatment. Food Reviews International. 2023; 40(6): 1796-1804. doi: 10.1080/87559129.2023.2238050
[2]Fabre JF, Niangoran NUF, Gaignard C, et al. Extraction, purification and stability of C-phycocyanin from Arthrospira platensis. European Food Research and Technology. 2022; 248(6): 1583-1599. doi: 10.1007/s00217-022-03987-z
[3]Ziyaei K, Abdi F, Mokhtari M, et al. Phycocyanin as a nature-inspired antidiabetic agent: A systematic review. Phytomedicine. 2023; 119: 154964. doi: 10.1016/j.phymed.2023.154964
[4]Sánchez-Laso J, Espada JJ, Rodríguez R, et al. Novel Biorefinery Approach for Phycocyanin Extraction and Purification and Biocrude Production from Arthrospira platensis. Industrial & Engineering Chemistry Research. 2023; 62(12): 5190-5198. doi: 10.1021/acs.iecr.2c03683
[5]Stanic-Vucinic D, Minic S, Nikolic MR, et al. Spirulina Phycobiliproteins as Food Components and Complements. Microalgal Biotechnology. 2018. doi: 10.5772/intechopen.73791
[6]İlter I, Akyıl S, Demirel Z, et al. Optimization of phycocyanin extraction from Spirulina platensis using different techniques. Journal of Food Composition and Analysis. 2018; 70: 78-88. doi: 10.1016/j.jfca.2018.04.007
[7]Anvar AA, Nowruzi B. Bioactive Properties of Spirulina: A Review. Microbial Bioactives. 2021; 4(1): 134-142. doi: 10.25163/microbbioacts.412117b0719110521
[8]Costa JAV, Freitas BCB, Rosa GM, et al. Operational and economic aspects of Spirulina-based biorefinery. Bioresource Technology. 2019; 292: 121946. doi: 10.1016/j.biortech.2019.121946
[9]Fu J, Huang Y, Liao Q, et al. Photo-bioreactor design for microalgae: A review from the aspect of CO2 transfer and conversion. Bioresource Technology. 2019; 292: 121947. doi: 10.1016/j.biortech.2019.121947
[10]Xu L, Weathers PJ, Xiong X, et al. Microalgal bioreactors: Challenges and opportunities. Engineering in Life Sciences. 2009; 9(3): 178-189. doi: 10.1002/elsc.200800111
[11]Zarrouk C. Contribution to the study of a cyanophycean (French) [PhD thesis]. University of Paris, Paris; 1966.
[12]Manav E. Investigation of parameters affecting biomass and metabolite production of cyanobacteria/microalgae in laboratory-scale photobioreactors (Turkish) [Master’s thesis]. Ege University, İzmir; 2004.
[13]Khazi MI, Demirel Z, Dalay MC. Evaluation of growth and phycobiliprotein composition of cyanobacteria isolates cultivated in different nitrogen sources. Journal of Applied Phycology. 2018; 30(3): 1513-1523. doi: 10.1007/s10811-018-1398-1
[14]Ajala SO, Alexander ML. Evaluating the effects of agitation by shaking, stirring and air sparging on growth and accumulation of biochemical compounds in microalgae cells. Biofuels. 2020; 13(3): 371-381. doi: 10.1080/17597269.2020.1714161
[15]Lu Z, Dai J, Zheng L, et al. Disodium 2-oxoglutarate promotes carbon flux into astaxanthin and fatty acid biosynthesis pathways in Haematococcus. Bioresource Technology. 2020; 299: 122612. doi: 10.1016/j.biortech.2019.122612
[16]Omidian K, Rafiei H, Bandy B. Increased mitochondrial content and function by resveratrol and select flavonoids protects against benzo[a]pyrene-induced bioenergetic dysfunction and ROS generation in a cell model of neoplastic transformation. Free Radical Biology and Medicine. 2020; 152: 767-775. doi: 10.1016/j.freeradbiomed.2020.01.021
[17]Isleten-Hosoglu M, Gultepe I, Elibol M. Optimization of carbon and nitrogen sources for biomass and lipid production by Chlorella saccharophila under heterotrophic conditions and development of Nile red fluorescence based method for quantification of its neutral lipid content. Biochemical Engineering Journal. 2012; 61: 11-19. doi: 10.1016/j.bej.2011.12.001
[18]Imamoglu E. Effect of Different Culture Media and Light Intensities on Growth of Haematococcus pluvialis. Int J Nat Eng Sci. 2007; 1(3): 5-9.
[19]Boussiba S. Carotenogenesis in the green alga Haematococcus pluvialis: Cellular physiology and stress response. Physiologia Plantarum. 2000; 108(2): 111-117. doi: 10.1034/j.1399-3054.2000.108002111.x
[20]Chaiklahan R, Chirasuwan N, Srinorasing T, et al. Enhanced biomass and phycocyanin production of Arthrospira (Spirulina) platensis by a cultivation management strategy: Light intensity and cell concentration. Bioresource Technology. 2022; 343: 126077. doi: 10.1016/j.biortech.2021.126077
[21]Hussin AA, To SW, Sani MH, et al. Optimisation and growth kinetic analysis of Microalgae, Arthrospira platensis in 2-L Photobioreactors. IOP Conference Series: Earth and Environmental Science. 2021; 842(1): 012036. doi: 10.1088/1755-1315/842/1/012036
DOI: https://doi.org/10.18686/ppas.v6i1.2257
Refbacks
- There are currently no refbacks.
