Intraperitoneal injection adenovirus pmultirnai-ldhc okay plateau pika (ochotona curzoniae) skeletal muscle ldhc gene of silence effect
Abstract
Intraperitoneal injection is a kind of simple and convenient to drug of style in order to verify intraperitoneal injection adenovirus Pmultirnai-ldhc. The plateau pika (Ochotona curzoniae) Skeletal Muscle Ldhc Gene Silence of feasibility will 27 Only plateau pika (Ochotona curzoniae) divided into interference group, Shell Group and blank control group each group9Only individual interference group and Shell Group respectively Injection0.65 mL Adenovirus Pmultirnai-ldhc And adenovirus Pmultirnai-NSBlank control group injected with the same amount of physiological saline injection after7/d Detection Skeletal Muscle in Ldhc Gene MRNA And protein of Expression Level Determination the lactate dehydrogenase (LDH) Activity and lactic acid (LD) And three phosphoric acid adenosine (ATP) Of content. Results show that and blank control group compared interference group in MRNA AND PROTEIN LEVEL, Ldhc Gene expression respectively reduce 41.73% And 15.76%; Lactate dehydrogenase (LDH) Activity, lactic acid (LD) And ATP Content respectively reduce 23.98%,51.08%And19.29%. Results (intraperitoneal injection adenovirus Pmultirnai-ldhcCan effective silence Skeletal Muscle in Ldhc Gene expression.
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DOI: https://doi.org/10.18686/ppas.v1i1.1148
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