Far north salamander digestive tract 6 of endocrine cells of immune organization chemical research

Abstract

Application protein-Biotin-Peroxidase complex (ABC) Immune organization Chemical Method of Far North salamander (Salamandrella Keyserlingii) Digestive Tract5-Serotonin (5-HT), Gastrin (Gas), Somatostatin (SS), Pancreatic hyperglycemia (GLU), People pancreatic polypeptide (PP) And P-Material (SP)6Of endocrine cells of distribution for the study. In this paper Duncan Multiple compare the of methods of endocrine cells in the digestive tract of distribution density the statistical analysis. Results display,5-Serotonin cells in digestive tract the parts were distribution, Gastric body (2.80 ± 0.70) And twelve duodenum (2.60 ± 0.75) Distribution density highest pylori minimum (0.85 ± 0.67); Gastrin cells only distribution in small intestine twelve duodenum (1.85 ± 0.75) Density highest; somatostatin cells distribution in esophageal, gastric body, pylorus, twelve duodenum and ileum the parts and pylorus (2.25 ± 0.64) Distribution density highest; pancreatic hyperglycemia of cells I saw in gastric cardia and gastric body people  pancreatic polypeptide Cells and P-Material cells in the whole digestive tract were not detection. Various endocrine cells of form diverse was round, oval, cone-shaped and spindle and. Far North salamander digestive tract endocrine cells of this a kind of density distribution and morphology characteristics and other amphibians and Heilongjiang origin far northern salamander compared both common and have difference, the reason may be and species, feeding habits and habitat environment and factors about.