RposEffects of gene deletion mutation on Stress Tolerance, quorum sensing and corruption activity of Pseudomonas fluorescens
Abstract
【Objective】The microbial activity is the main cause of food corruption. It is of great significance to study the regulation mechanism of food spoilage bacteria for ensuring food quality and safety. Pseudomonas fluorescens is a representative spoilage bacteria in food.RNASelectivity of PolymeraseSigmaFactorRposThe role of Pseudomonas fluorescens in the Process of corruption. [Methods] The homologous recombination method was used to construct the isolate of Pseudomonas fluorescens frozen fish.RposThe survival rate of wild-type and mutant were compared under different stress conditions.-Tandem Mass SpectrometryLC-MS MS)Analysis of the production of Homoserine esters by wild-type and mutant strains(AHLs)Type and content of quorum sensing signal molecules; detection of wild-type and mutant strains inoculated in sterilized salmon sauce4 °CThe total number of colonies and the production of volatile basic nitrogen during storage. [Results] Pseudomonas fluorescens was successfully constructed.RposGene Deletion Mutant.RposGene Deletion leads to10 mmol/L H2.O2.And15%Ethanol tolerance was significantly reduced.150 g/mL?Crystal purple and175 mmol/LThe tolerance of acetic acid to a certain extent, does not affect its47 °CAnd20% NaClTolerance. Pseudomonas fluorescensRposLong Chain signaling molecules after gene deletion and mutationC10-HSL,C12-HSLAndC14.-HSLThe content increased. Detection of spoilage activity in sterilized salmon sauceRposGene Deletion resulted in a significant decrease in the production of volatile basic nitrogen in Pseudomonas Fluorescens. [Conclusion] Pseudomonas fluorescensRposNot only regulate bacterial tolerance to various stress conditions, but also affectAHLQuorum Sensing and corruption.
Keywords
References
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