Detection of serum bisphenol A with enzyme-linked
Abstract
To establish an indirect competitive enzyme-linked immune assay (IC-ELISA) usingMonoclonal Antibody of bisphenol A (BPA) for the Detection of Serum BPA.MethodsTotally 176 puils aged 8-10 years were selected from three primary schools in suburban area of Nanchang city of Jiangxi Province and Serum bpa of the puils were determined with IC-ELISA established.ResultsThe detection limit of the established IC-ELISA method was 0.43 ng/ml. The recovery rates of BPA in serum spiked at 4 different levels were between 70.1%And 87.8%With the variation coefficients from 4.79%To 9.41%. BPA was detected in 95 of the 176 serum samples and the detection rate was 54%. The Serum BPA content ranging from non-detectable to 26.48 ng/ml for all the samples. the detection rate of Serum BPA was higher along the boy puils than the girl puils, but the detection rate was not significant difference (P= 0.195 ).ConclusionThe established IC-ELISA could meet the requirement of Serum BPA detection along children. BPA was detectable in serum of Children in Nanchang city of Jiangxi Province and relatively higher Serum BPA content was detected in some of the children, suggesting studies are needed to explore the contactsource of BPA.
KeyWords: Enzyme-linked immune assay; serum bisphenol A; Children
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