Construction, immunogenicity study. recombinant Ad5 vaccine expressing HIV-1 CRF01_ AE gag gene
Abstract
Objective To construct recombine AD5 Vaccine Encoding codon-optimized HIV-1 crf01_ AE gag gene and evaluate Its Immunity in Mice.Methods The HIV-1 crf01_ AE gag gene was optimized in the previous phase of our laboratory,A combined AD5 vaccine carrying the gag gene,RAd5-HIV aegag,Was connected.After confirmation that the combined AD5 vaccine ould express Gag protein,The immune effect of recombine vaccine rAd5-Hivaegag was evaluated in BALB/c mice.: Recombinant Ad5 vaccine expressed Gag protein efficiently, induced high level. humoral, cellular immune responses. immunized BALB/c mice.Conclusion. recombinant Ad5 Vaccine Encoding HIV-1 CRF01 of AE gag gene. constructed successfully.
Keywords
Full Text:
PDFReferences
Maartens GCelum CLewin SR.HIV infection:EpidemiologyPathogenesisTreatmentAndprevention[J].The Lancet2014 384(9939):258-271.
Williams WBLiao HXMoody MAEt al.HIV-1 vaccines.Diversion HIV-1 vaccine-inducedimmunity by gp41-microbiota cross-reactive antibodies[J].Science2015349(6249):1253.
Ferrando-Martinez SCasazza JPLeal MEt al.Differential Gag-specific polyfunctional T cell maturation patterns. HIV-1 elite controllers[J].J Virol201286(7/):3667-3674
Han XAn MZhang MEt al.Identification. 3 distinct HIV-1 founding strains responsible forexpanding epidemic among men who have sex. men 9 Chinese cities[J].J acquir Immune deficsyndr201364(1):16-24.
Wu JMeng twig u & ZXu JEt al.New emerging recombinant HIV-1 strains, close transmission linkage HIV-1 strains. Chinese MSM population indicate a new epidemic risk[J].PLoS ONE20138(1):54322.
Ondondo BO.. Influence. delivery vectors. HIV Vaccine efficacy[J].Front Microbiol2014(5):439
Graziani GMAngel JB.Evaluating. efficacy. Therapeutic HIV vaccines through analytical treatmentinterruptions[J].J Int
Chen Dan ying what Cho, Wang Meng ran and.With codon OptimizationSIV gagGeneDNAVaccine andRAd5Vaccine construction and immunogenicity of evaluation [J].Chinese experimental and clinical virology Journal,201428(1):17-19.
More than Refining.ExpressionSIVAndHIVGene of multi-carrier Vaccine Combined with application of immune PolicySlightly and related adjuvant study [D]., Beijing, Industrial University in,2016.
Yang kai Liu Cong Chen Hui Ping and.45CasesHIVAntibody don't another person follow-up detection results analysis [J].Public Health and Prevention medical,201627(04):107-109.
Refbacks
- There are currently no refbacks.
