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Based onMBRMicrobial Community Structure Analysis of shortcut nitrification initiation in different kinds of mud

Huang Dianxia, Ziming Li


 For clear membrane bioreactor (MBR)Microbial Community structure changes before and after the short-cut Nitrification with different sludge inoculation.MBRNitrifying sludge (R1.),Anaerobic nitrification sludge (R2.)And1.:1.Mixed inoculation of anaerobic and Denitrifying sludge (R3.)Sludge source for achieving rapid short-cut nitrification.The results show that the combination of Intermittent Aeration and reduced hydraulic retention time (HRT),R1.R2.WithR3.Reactor time-consuming46 d,8 dAnd30 dThe shortcut nitrification was successfully launched,R2.Shortest startup cycle of Reactor.During the stable operation period,R1.R2.AndR3.The average nitrite accumulation rate was92%,93%And94%,R3.The reactor showed more stable short-cut Nitrification performance..Ace,Chao,ShannonAndSimpsonIndex results show that after stable operation,R1.AndR2.The microbial abundance and diversity of the reactor were significantly lower than that of the inoculated sludge,R3.The abundance of reactor species decreased slightly while the diversity level changed little..After the shortcut nitrification was successfully started,3.The main bacteria in the reactor were Proteus phyla (Proteobacteria)And bacteriocin (Bacteroidetes)And the abundance of the Main Nitrogen Removal Function bacteria is higher than that of the inoculated sludge..Beta-Proteus3.The dominant bacteria in the short-cut Nitrification system of each reactor accounted59. 6%,63. 6%And69. 3%.R1.R2.AndR3.Reactor in the advantage bacteria of are nitrosation single (Nitrosomonas)Of proportion respectively up12. 8%,Natural 20. 2%And19. 7%.ComparedR1Reactor,R2AndR3Reactor inoculation sludge in there is a certain proportion of nitrification bacteria more conducive to system short-range nitrification of implementation.


short-range nitrification; Membrane Biological Reactor; inoculation sludge; start; microbial community structure

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Volcke e I,Van hulle S W,Donckels B m,Et al.Coupling Sharon process with ANAMMOX: Model-based Scenario Analysis With Focus On Operating Costs[J].Watern Science AndTechnology,2005,52(4.):107-115.

Yu yingcui, tall Wen, Tao Chong, et al.Start-up of ANAMMOX using sequencing batch biofilm reactor [J [J].China Environmental Science,2012,32(5.):843-1-849.

Yu Y C,Gao D W,Tao y,Et al.Start-up of ANAMMOX inSequencingBatchBiofilmReactor(SBBR)[J].ChinaEnvironmental Science,2012,32(5.):843-849.

Lackner s,Gilbert E M,Vlaeminck S E,Et al.Full-scale partial nitritation/ANAMMOX experiences-An Application Survey[J].WaterRSearch,2014,55:292-303.

Zhang Liang.Biological Nitrogen Removal Process and optimization control of high ammonia nitrogen sludge digestion liquid [J [D].Harbin: Harbin Institute of Technology,2013.42-43.

Zhang L.Development and optimization of the nitrogen removal of high ammonium sludge dewatchering water[D].Harbin:Harbin Institute of Technology,2013.42-43.

Tan N C GKampschreur M JWanders WEt al.Physiological, phylogenetic study, ammonium-oxidizing culture at high nitrite concentrations[J].Systematic, Applied Microbiology

Harris c a Routledge e j Schaffner C et al. benzotri-azole. antiestrogenic. vitro but not in vivo [J]. environ-mental Toxicology, Chemistry 2007 26 (11): 2367-2372

He T Liang B Liu W H et al. estrogenic potential. benzotriazole. marine medaka (Oryzias melastigma) [J]. ecotoxicology, environmental Safety 2012 80: 327-332

Liang x f Wang M Chen X et al. endocrine disrupting effects. benzotriazole. rare minnow (gobiocypris rarus). a sex-dependent manner [J]. chemosphere 2014,112: 154-162

Liang x Li J martyniuk c j et al. benzotriazole ultravi-olet stabilizers alter. EXPRESSION. thyroid hormone pathway. Zebrafish (Danio rerio) embryos [J]. chemo-sphere 2017,182: 22-30

Filimonov D a lagunin A gloriozova t a et al. pre-diction. Biological Activity spectra. organic com-pounds using. PASS online web resource [J]. chemis-Try. Heterocyclic compounds 2014 50 (3): 444-457

Lagunin A stepanchikova A filimonov D et al. PASS: prediction. activity spectra. biologically active sub-stances [J]. Bioinformatics 2000 16 (8): 747-748

Takahashi I ojima N Ogura K et al. Purification Characterization of dimetallyl pyrophosphate-aspulvi-None dimetallytransfer from Aspergillus terreus [J]. Biochemistry, 1978, 17 (13): 2696-2702



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