Metagenomics Study on the Effect of β-lactam Antibiotic on the Structure of Rumen Bacteria in Calves

Abstract

(Objective) This paper aims to study the effect of β-lactam antibiotic on the structure of rumen bacteria in calves by metagenomics. (Method) In this experiment, 54 newborn calf were selected and randomly divided into 3 treatments according to the principle of similar birthday age. The test was divided into: 1) control group (CK), feeding no antibiotic milk group; 2) antibiotic milk group (AM); 3) pasteurization antibiotic milk group (APM). The trial period was 180 days. The rumen bacteria genomic library of calf was constructed from 60 g (T1), 90 d (T2) and 180 d (T3) calf rumen fluids in each group. (Results): The results indicate that (1) The high-quality reads of the nine samples accounted for more than 96% of the percentage of reads and high-quality sequence bases. (2) The reads bases obtained were in the 10%-90% range and the Q value was in the 35-40 range. (3) The bacteria with the highest abundance of 9 samples were Bacteroidetes (average abundance=69.8%), followed by Firmicutes (average abundance=9.8%). (Conclusion) Through screening, it was determined that the two bacteria of the rumen of the calf rumen played an important role in the digestion of nutrients in the r umen. Bacteroides for pasteurized milk containing β-lactam antibiotics More sensitive. The antibiotic milk with β-lactam antibiotic may cause food safety related drug resistance problems by affecting spirulina bacteria.