REplication jumping duration PCRAmplification,Closing and Sanger sequencing of a fragment Of the temptation gene in black-spotted frog1
Abstract
During in vitro replication of DNA by DNA Polymerase,The diverse palindromic StructuresOn templates of DNA can lead to replication jumping and cause fragments to be missing from daughter strands.We recorded replication jumping in which a 564 bp fragment was missing from daughter DNA.The original template was a 1126 bp fragment of the introduction of the temporary gene of black-spotted frog(Pelophylax nigromaculatus).The replication jump was recorded after amplifica-tion of this template using polymerase chainREaction,Closing Using Vector,And sequencing using the Sanger Method.The replication jump was elevated to rise from this series of events:
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DOI: https://doi.org/10.18686/pas.v1i1.1172
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