Establishment of metaasis lung cancer model by injection Of EMT-6 cells in BALB/c mice

Abstract

Objective To establish a metaasis lung cancer model with parent Clinic,Stable and repeatable charac-Carcinoma at logarithmic growth phase were harvest by 0. 25% trypsin digestion,And equal rated at 3 dens of 1x106.Cells per ml(High Concentration),5x105.Cells per ml(Medium concentration),And 1x105.Cells/mL(Low Concentration). Phosphate-buffered saline., then 0. 2 mL EMT-6 tumor cells. intravenously injected. evalua-tion indicators, biological characteristics, As clinic appearance. Time. tumor formationSurvival time. Tumor growth, pathological characteristics. metastasis lung models. different concentrations. then studied. screen. best metastasis lung cancer model. Finally. Repeatability, stability. model. determined by 3 repeated exper-iments. successfully establish. metastasis lung cancer model. BALB/c mice.REsults BALB/c mice. anatomized after EMT-6 tumor cells Injection,: Showed,. group. high concentration treatmentTumor nodes. lung surface began. appear at day 7/, all animals died 18 d;In the group of medium concentration,Tumor nodes on Lung surface begin to appreciate at day 7 and all animals divided in 28 d;In the group of Low Concentration,Tumor nodes on lung surface appeared partially at day 14,All mice lung surface had tumors at day 21,The number of tumors reach the highest level(An average of 12-15/mouse),The volume of tumors' became much bigger at day 35,Mice began to die at day 24,And all mice divided in 42 d. Conclusion we excluded that the establishment of mice models using low EMT-6 cell concen-tration can provide convenient 4-week time window for treatment,Observation and test from tumor formation to dead,The histo-pathology of models 'lung was consistent with clinical features of Lung Cancer,The clinic appearance was easy to identify,And the repeatability and stability tests the models were consistent along the 3 times.