Nile tilapiaXbp1-SGene Cloning and Expression Analysis
Abstract
To learn about Nile tilapia(Oreochromis niloticus)Cell Transcription FactorXbp1-S(OnXbp1-S)Sequence characteristics of the Gene and Its Role in Streptococcus agalactiae(Streptomyces alactolyticus)Stress and inBRole in cell differentiation, ApplicationRaceObtained by cloning technologyOnXbp1-SFull Length of Gene1380 BPIncluding open reading frameORFFor1155 BP,5.Wang YiTerminal non-coding region(5)Wang YiUTR)
The organization in have expression,MRNALevel on in liver in expression highest protein level in thymus in expression highest and in muscle in expression minimum. No milk Streptococcus stress after,OnXbp1-SGene in liver and spleen in expression trend similar were in stress during appear expression was up-regulated in192 hThe peak. In addition immune was analysis found,OnXbp1-SFactor in different differentiation degreeBCells subclass in Expression Difference in matureBCells in present high expression and in not matureBCells in almost don't expression. Research results show that,OnXbp1-SParticipate in Nile tilapia on no milk streptococcus of immune defense and inBCells Differentiation role. This study will for further studyOnXbp1-SFactor response pathogenic bacteria infection of mechanism and promoteBCells Differentiation Mechanism provide theory basis.
Keywords
Full Text:
PDFReferences
Calfon M Zeng H Urano F,Et al. IRE1 couples endoplasmic reticulum load. secretory capacity by processing. XBP-1 mRNA. Nature, 2002,415 (6867): 92-96
FangeR. Lymphoid System organs. sturgeons (acipenseridae). Veterinary Immunology, immunopathology 1986 12 (1-4): 153-161
Gan twig u & Z Wang B Lu Y,Et al. Molecular Characterization, EXPRESSION. LCK. Nile tilapia (Oreochromis niloticus) In responseStreptomyces agalactiaeStimulus. applied biochemistry and biotechnology, 2015,175 (5)
Hu MC, Gong hy, Lin GH,Et al. XBP-1, a key regulator of unfolded Protein Response, activate transcription of IGF1 and Akt Phosphorylation in zebrafish embryonic cell line. Biological and Biological Research Communications, 2007,359 (3): 778-783
Iwakoshi NN Lee ah, glimcher LH. the X-Box Binding Protein-1 transcription factor is required for plasma cell differentiation and the unfolded protein response. immunohistochemical reviews, 2003,194 (1): 29-38
Iwakoshi NN pypaert M Glimcher LH. Transcription Factor XBP-1. essential. development, survival. dendritic. Journal. experimental Medicine 2007,204 (10): 2267-2275
Kaattari SL Irwin MJ. salmonid spleen, anterior kidney harbor populations. lymphocytes. different B cell repertoires. developmental, comparative Immunology 1985 9 (3): 433-444
Leong JS jantzen SG von schalburg KREt al.Salmo salarEsox luciusFull-length cDNA sequences reveal changes.Evolutionary pressures. a post-tetraploidization genome. BMC Genomics 2010 11 (1): 279
Liou HC Boothby MR Finn PW,Et al. A new member. leucine zipper class. proteins for, binds. hla dr Alpha promoter. Science, 1990,247 (4950): 1581-1584
Lu MX. Review. research. streptococcosis. tilapia. South; Fisheries Science 2010 6 (1): 75-79 [Lou maxiem.Tilapia Streptococcus Disease Research Progress.South China Fisheries2010 6 (1): 75-79]
Ma C ye J kaattari SL. Differential compartmentalization. Memory B cells versus plasma cells. salmonid fish. European Journal. Immunology 2013 43 (2): 360-370
Ohtani M miyadai T hiroishi S. Identification. genes Encoding critical factors regulating B-cell terminal differentiation. torafugu (Takifugu rubripes). Comparative Biochemistry, Physiology Part D genomics proteomics 2006 1 (1): 109-114
Reimold AM iwakoshi NN Manis J,Et al. Plasma cell differentiation requires. transcription factor XBP-1. Nature, 2001,412 (6844): 300-307
Robinson AS Hines V Wittrup KD. Protein Disulfide Isomerases overexpression increase secretion. foreign protein.Saccharomyces cerevisiae. Biotechnology 1994 12 (4 ):381-384
Salinas I zhang ya Sunyer JO. Mucosal immunoglobulins, B cells. teleost fish. developmental, comparative Immunology 2011 35 (12): 1346-1365
Tellez.-Ba n uelos MC Santerre A Casas-Solis J,Et al. Oxidative stress. macrophages from spleen. Nile tilapia (Oreochromis niloticus) Exposed. sublethal concentration. endosulfan. Fish, Shellfish Immunology 2009 27 (2): 105-111
DOI: https://doi.org/10.18686/fsa.v1i1.1207
Refbacks
- There are currently no refbacks.
