Study on Insitu Bioremediation of Cr(VI) in Porous Media

Wenjing Niu, Zhendong Fang, Hua Zhong

Abstract


Indigenous microorganism that show an ability for Cr (VI) reduction were isolated from a chromium-contaminated sediment. Sand column experiments were conducted using the isolated bacteria to investigate microorganism effects on Cr(VI) reduction in closed systems that simulated subsurface conditions. The results showed that when glucose and emulsified oil were used as carbon sources, the effective reduction of Cr(VI) can be achieved, and the reduction rate of Cr(VI) increases with the increase of carbon source. The reduction rate of Cr(VI) decreases with the increase of initial Cr(VI) concentration, which is consistent with the first-order reaction kinetics. The reaction rate constant decreases with the increase of initial Cr(VI) concentration. The reduction rate of Cr(VI) increased with the increase of the hydraulic residence time, that is, the emulsified oil has relatively high stability and low biodegradation rate, and can provide carbon source in the bioreduction process for a long time.


Keywords


Indigenous Microorganism; Cr(VI) Reduction; Column Experiment

Full Text:

PDF

Included Database


References


Wang Aiyun, Zhong Guofeng, Xu Gangbiao, et al. Effects of Chromium Stress on Physiological Characteristics and Chromium Accumulation of Brassica juncea. Environmental Science. 2011, 32(6).1717-1725.

Gonnelli C, Renella G. Chromium and Nickel. 2013

Cheung K H, Gu J D. Mechanism of hexavalent chromium detoxification by microorganisms and bioremediation application potential: A review. International Biodeterioration & Biodegradation. 2007, 59(1): 8-15.

Park D, Yun Y S, Cho H Y, et al. Chromium Biosorption by Thermally Treated Biomass of Brown Seaweed Ecklonia sp. Industrial & Engineering Chemistry Research. 2013, 43(26): 8226-8232.

Yang Zhihui, Wu Ruiping, Wang Bing, et al. Microbial Remediation of Chromium Contaminated Soil and Pilot Test. Environmental Chemistry. 2013, 32(9): 1758-1765

Abhipsa S, Chandraraj K. Comparison of in vitro Cr(VI) reduction by CFEs of chromate resistant bacteria isolated from chromate contaminated soil. Bioresource Technology. 2008, 99(10): 4130-4137.

Liu Y G, Xu W H, Zeng G M, et al. Cr(VI) reduction by Bacillus sp. isolated from chromium landfill. Process Biochemistry. 2006, 41(9): 1981-1986.

Mangaiyarkarasi M S M, Vincent S, Janarthanan S, et al. Bioreduction of Cr(VI) by alkaliphilic Bacillus subtilis and interaction of the membrane groups. Saudi J Biol Sci. 2011, 18(2): 157-167.

Camargo F A O, Okeke B C, Bento F M, et al. Diversity of chromium-resistant bacteria isolated from soils contaminated with dichromate. Applied Soil Ecology. 2005, 29(2): 193-202.

Megharaj M, Avudainayagam S, Naidu R. Toxicity of hexavalent chromium and its reduction by bacteria isolated from soil contaminated with tannery waste. Current Microbiology. 2003, 47(1): 51-54.

Francisco R, Alpoim M C, Morais P V. Diversity of chromium-resistant and -reducing bacteria in a chromium-contaminated activated sludge. Journal of Applied Microbiology. 2010, 92(5): 837-843.

Bopp L H, Ehrlich H L. Chromate resistance and reduction in Pseudomonas fluorescens strain LB300. Archives of Microbiology. 1988, 150(5): 426-431.

Deleo P C, Ehrlich H L. Reduction of hexavalent chromium by Pseudomonas fluorescens LB300 in batch and continuous cultures. Applied Microbiology & Biotechnology. 1994, 40(5): 756-759.

Myers C R, Carstens B P, Antholine W E, et al. Chromium(VI) reductase activity is associated with the cytoplasmic membrane of anaerobically grown Shewanella putrefaciens MR-1. Journal of Applied Microbiology. 2010, 88(1): 98-106.

Middleton S S, Rizlan Bencheikh L, Mackey M R, et al. Cometabolism of Cr(VI) by Shewanella oneidensis MR-1 produces cell-associated reduced chromium and inhibits growth. Biotechnology & Bioengineering. 2003, 83(6): 627-637.

Mabbett A N, Macaskie L E. A novel isolate of Desulfovibrio sp. with enhanced ability to reduce Cr(VI). Biotechnology Letters. 2001, 23(9): 683-687.

Horton R N, Apel W A, Thompson V S, et al. Low temperature reduction of hexavalent chromium by a microbial enrichment consortium and a novel strain of Arthrobacter aurescens. Bmc Microbiology. 2006, 6(1): 1-8.

Megharaj M, Avudainayagam S Naidu R. Toxicity of hexavalent chromium and its reduction by bacteria isolated from soil contaminated with tannery waste. Current Microbiology. 2003, 47(1): 51-54.

Alam M, Hossain M A, Yonge D R, et al. Bioreduction of Hexavalent Chromium in Flow-Through Quartz

Sand Columns. Journal of Environmental Engineering.

Kumari D, Pan X, Zhang D, et al. Bioreduction of Hexavalent Chromium from Soil Column Leachate by Pseudomonas stutzeri. Bioremediation Journal. 2015, 19(4): 249-258.

Tal B M, Ishai D, Brian B. Transport of metal oxide nanoparticles in saturated porous media. Chemosphere. 2010, 81(3): 387-393.

Liu Y G, Xu W H, Zeng G M, et al. Cr(VI) reduction by Bacillus sp. isolated from chromium landfill. Process Biochemistry. 2006, 41(9): 1981-1986.




DOI: https://doi.org/10.18686/pes.v2i1.1308

Refbacks

  • There are currently no refbacks.